Yes, you can.
The most important thing to know is that user running windows service where pdfprint is used must have rights to print to selected printer - otherwise it will not work.
Set up windows service to be run as a user account type:
User account type - causes the system to prompt for a valid user name and password when the service is installed and runs in the context of an account specified by a single user on the network
If you want to start windows service as local system account, than system local account must have printer rights.
NOTE: Following instruction were found at: http://support.microsoft.com/kb/184291
To set up printers for the SYSTEM account, perform the following:
This method requires you to modify the registry using the Registry Editor.
WARNING: Using Registry Editor incorrectly can cause serious, system-wide problems that may require you to reinstall Windows to correct them.
Microsoft cannot guarantee that any problems resulting from the use of Registry Editor can be solved.
Use this tool at your own risk.
Ensure that the user you are currently logged into on the server has the desired printers installed.
Launch the Registry Editor (Regedit.exe).
Select the following key:
HKEY_CURRENT_USER\Software\Microsoft\Windows NT\Current Version\Devices
From the Registry menu, click Export Registry File.
In the File Name text box, type c:\Devices.reg.
Select the following key:
HKEY_CURRENT_USER\Software\Microsoft\Windows NT\Current Version\PrinterPorts
From the Registry menu, click Export Registry File.
In the File Name text box, type c:\PrinterPorts.reg.
Select the following key:
HKEY_CURRENT_USER\Software\Microsoft\Windows NT\Current Version\Windows
From the Registry menu, click Export Registry File.
In the File Name text box, type c:\Windows.reg.
From the Start button, select Run. Open Devices.reg in Notepad by typing Notepad Devices.reg in Run dialog box.
Replace the text HKEY_CURRENT_USER with HKEY_USERS\.DEFAULT
Save the file. Then import it into the registry by double-clicking the file in Windows Explorer.
Repeat steps done for Devices.reg for PrinterPorts.reg and Windows.reg.
These steps only work for local printers.
If you are using PrintWithAdobe method, please also read
Can PdfPrint use Adobe for silent printing?